![]() ![]() The levels of study elements and folate in AF and MS in groups with abnormalities were compared with healthy deliveries as previously published. The values of recovery are within an acceptable range for all analytes, which demonstrates that the described analytical procedure is fit for the intended purpose. The trueness of the analytical method was assessed by analyzing the certified reference material (CRM) Seronorm™Trace Elements Serum L-2. The linearity-calculated as R 2-was acceptable for all analyzed elements ( R 2 > 0.999). Calibration based on a weighted least squares calibration curve was employed for all elements. ![]() An Elan DRC II ICP-MS (PerkinElmer SCIEX, Ontario, Canada) was used to determine: Mg, Co, Cu, Zn, Sr, Cd, Ba, Pb, U, Ca, Cr, Al, Mn, V, and Fe in AF and MS. Samples were mineralized in a high-pressure, closed, microwave digestion system (Ethos One, Milestone). Measurement of essential and toxic elements in AF and MS has been described by Markiewicz et al. Whole blood hemolysate (NIBSC code: 95/528) served as an external quality control the measured mean folate concentration was 12.8 ng/mL (target value 13 ng/mL) with an inter-assay variation of 7.8%. The inter-assay variations for serum folate concentrations was 5.9%. We used chloramphenicol-resistant Lactobacillus casei as the test microorganism. The results of this exploratory study indicate that the levels of essential and toxic elements are associated with fetal and newborn anatomical abnormalities and growth disorders.įolate analysis in AF and MS was performed according to the method described in our previous article. An inductively coupled plasma mass spectrometry technique was used to determine the levels of various elements in AF and MS. AF and MS were collected during vaginal delivery or during cesarean section. A total of 258 pregnant Polish women in the age group of 17–42 years participated in this study. We determined the association between essential and toxic elements and the level of folate in maternal serum (MS) and amniotic fluid (AF), along with neonatal abnormalities. The relevance of essential and toxic elements on fetal development has not yet been fully investigated, and the results of recent research indicate that these elements may be crucial in the assessment of the risk of malformations in neonates. ![]() Several studies have shown that genetic, nutritional, and environmental factors may have an impact on fetal development and neonatal health. Congenital birth defects may result in a critical condition affecting the baby, including severe fetal/neonatal handicap and mortality. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |